CBSE 2013 Bio-Technology Question Paper for Class 12

About this paper

This is the CBSE Class 12 Bio-Technology question paper code 99 (Series SKS) from the 2013 board examination, printed in both Hindi and English. It carries 70 marks over three hours and has 28 compulsory questions in four sections, rising from one-mark very short answers to three five-mark long answers. There is no overall choice, but internal choices are given in one three-mark question and two five-mark questions. Questions range across cell and tissue culture, recombinant DNA work, protein studies and genomics, including a numerical on how long two 50 L fermentors need to produce 500 g of erythropoietin. Calculators are not allowed; log tables may be used. This copy has the questions only, without answers.

Exam

CBSE Class 12

Subject

Bio-Technology

Year

2013

Paper code

99

Maximum marks

70

Time allowed

3 hours

Questions

28

Pages

7

Answers included

No, questions only

Paper pattern

• Section A

  5 marks

  Questions 1–5, very short answers, one mark each

• Section B

  20 marks

  Questions 6–15, short answers, two marks each

• Section C

  30 marks

  Questions 16–25, short answers, three marks each; internal choice in one

• Section D

  15 marks

  Questions 26–28, long answers, five marks each; internal choice in two

Topics covered

• Microbial culture and growth kinetics
• Plant cell and tissue culture
• Animal cell culture and cell lines
• Recombinant DNA technology and cloning vectors
• Transgenic plants and animals
• Protein engineering and downstream processing
• Mass spectrometry and protein analysis
• DNA fingerprinting and Southern hybridisation
• DNA sequencing and microarrays
• Monoclonal antibodies
• Bioinformatics and sequence databases

Questions from this paper

1. In large-scale microbial culture media, olive oil or sunflower oil is added. What purpose does this serve?
2. Give one reason why computational methods of counting the genes in a genome are inaccurate.
3. A DNA fragment of 22 kbp has to be cloned. Indicate, with reasons, the cloning vector and host that can be used.
4. During downstream processing of proteins, the pH is kept constant, processing time is kept to a minimum and agitation of the solutions is avoided. Suggest a reason for each.
5. In a pilot experiment, CHO cell lines expressed erythropoietin at 100 mg per 500 mL of culture medium. A company must produce 500 g of this protein using two 50 L fermentors, each of which can run only once a week. How long will it take?
6. The microarray technique can be used to compare gene products in normal and cancer cells. Explain the principles involved, with a relevant diagram.