CBSE 2015 Bio-Technology Question Paper for Class 12

About this paper

This is the CBSE Class 12 Bio-Technology question paper code 99 (Series SSO, Set 4) from the 2015 board examination, printed in both Hindi and English. It carries 70 marks over three hours and has 28 compulsory questions in four sections: six one-mark questions, eight two-mark, eleven three-mark and three five-mark questions. There is no overall choice, but internal choices are given in one two-mark question and two five-mark questions. Questions cover animal and microbial culture, cloning enzymes, Agrobacterium-mediated gene transfer, edible vaccines, molecular pharming, FISH, mass spectrometry and bioinformatics databases, with a long answer on nutraceutical proteins and probiotics as one option. Calculators are not allowed; log tables may be used. This copy has the questions only, without answers.

Exam

CBSE Class 12

Subject

Bio-Technology

Year

2015

Paper code

99

Maximum marks

70

Time allowed

3 hours

Questions

28

Pages

9

Answers included

No, questions only

Paper pattern

• Section A

  6 marks

  Questions 1–6, very short answers, one mark each

• Section B

  16 marks

  Questions 7–14, short answers, two marks each; internal choice in one

• Section C

  33 marks

  Questions 15–25, short answers, three marks each

• Section D

  15 marks

  Questions 26–28, long answers, five marks each; internal choice in two

Topics covered

• Protein purification and downstream processing
• Animal cell culture
• Microbial culture
• Plant tissue culture and embryo rescue
• Recombinant DNA technology and cloning enzymes
• Protein engineering
• Transgenic plants and edible vaccines
• Molecular pharming
• Genomics and gene prediction
• DNA sequencing and SNPs
• Bioinformatics databases

Questions from this paper

1. What is the relationship between the specific activity and the purity of a protein?
2. Why is r-HuEPO preferred over blood transfusion for people who have lost blood?
3. Why is an inverted microscope used instead of a compound microscope to observe animal cells in culture?
4. Describe how Agrobacterium tumefaciens can be used to introduce a foreign gene into plants.
5. Several concerns are being raised about accepting transgenic crops. List any six of them.
6. Explain, with a suitable diagram, the principle and steps of Sanger's method of DNA sequencing.